来自加拿大代写，的顾客授权发布的fundamentals of neuroscience，BIOL2260作业要求片段，我们不会发布BIOL2260的answer在网站，我们曾经写过BIOL2260及相关的fundamentals of neuroscience写过很多作业，考试，如果你也需要代写这个课程的作业请联系客服WX:QQ 5757940 ，代写人的代写服务覆盖全球华人留学生，可以为CA的学生提供非常准时精湛的服务，小作业assignment代写、essay代写享适时优惠，project、paper代写、论文代写支持分期付款，网课、exam代考预约时刻爆单中赶紧来撩。
Your Assignment: The big question is: What type of ion channels are the MACs in our neurons? Are they selective and permeable for only one ion species, or are they unselectively permeable for several different cation and/or anion species? If they are indeed selectively permeable for only one specific ion species which one is the most likely candidate?
How can you tell this by analyzing the provided information (data from our experiments)?
The big question is: What type of ion channels are the MACs in our neurons? Are they selective and permeable for only one ion species, or are they unselectively permeable for several different cation and/or anion species? If they are indeed selectively permeable for only one specific ion species which one is the most likely candidate?
How can you tell this by analyzing the provided information (data from our experiments)? Your job is to
I) formulate a hypothesis describing what type of ion channel we can expect our MACs to be, and reasonably explain why and how you come to this conclusion (10 points), and
II) think about, describe, explain, and discuss a possible strategy for a follow‐up experiment that we could do to verify your hypothesis (4 points). For part II keep in mind what factors determine an ion’s equilibrium potentials and how we could use this to test your hypothesis.
In addition, answer the following questions that may help to complete I) and II):
III) Looking at the results (Figure 1, Table 2) describe what forces drive the ion flow across the membrane through open MACs at ‐ 100 mV, ‐50 mV, 0 mV, +50 mV, and +100 mV. How does changes in VM determine the direction of ion flow across the membrane, i.e., the membrane current we measured (see Figure 1 and Table 2)? (4 points)
IV) Imagine doing this experiment without voltage‐clamp, i.e., have the cell at its natural membrane resting potential of VM = ‐55.7 mV when starting stimulation and ions crossing the membrane through open MACs carrying a membrane current. How would the flow of ions across the membrane (and the current carried by those ions) affect the membrane potential VM under non‐voltage‐clamp conditions? Will VM change or not, and if it changes will VM become more negative (hyperpolarize) or less negative (depolarize)?
Explain your answer! (4 points)
V) Will the results of this experiments change if it is conducted at 10 °C? What parameters will change, how does this affect the observed current, and would the change require you to review your hypothesis under I) and follow‐up experiment II)? (3 points)
Sounds though? Think about what you learned in class and use it to interpret information can be read out of Figure 1 and Table 2. Ask yourself the following questions before writing up your assignment. Think before you write, and proofread not only for spelling and grammar, but also for logical flow in your argumentation.
What forces drive the movement of ions across the cell membrane through open ion channels (here MACs) and determine the direction of ion movement at different VM?
What ion species (Na+, K+, Ca2+, Mg2+, or Cl‐) can carry the observed current across the cell membrane at the given VM, i.e., move in the required direction across the membrane at the different VMs to result in the observed current IR?
Why did we care about calculating and knowing equilibrium potentials prior to our experiments?
How can we prevent ions from flowing through open ion channels or speed‐up their flow in experiments like this one?
What will happen if we change the extracellular concentration of one ion species? Will this change the resulting membrane current carried by this ion species and the resulting data plot?
MSVU BIOL/PSYC2260 Copyrighted Material for Class Use Only! No Unauthorized Distribution or Publication!
The completed Assignment must be uploaded to Moodle as Word or PDF file and meet the following formal requirements:
No photos or scans of handwritten material, even if embedded in a Word or PDF file, will be accepted for evaluation.
Font type Times New Roman, Calibri, or Arial in Size 12 pt., Double Line Spacing, 2.5‐inch (6 cm) Border on the Right‐Hand Side (to provide room for comments and remarks) and a 1‐inch (2.5 cm) Border on the Left‐Hand Side of each page.
Text must be properly formatted, e.g., Na+ not Na+ or sodium. Working with text processors and editing software is a required “soft skill” that is expected from academics.
Submitted files must be named in the format YOUR FIRST NAME_YOUR LASTNAME (e.g., jane_doe.doc or john_doe.pdf).
Your FULL NAME must be in the document as part of the text (e.g., as title “Assignment #1 by Jane Doe”)!
Failure to Comply with These Formal Requirements will lead to a 10 %‐Deduction (‐1.5 pts = max 13.5 towards the Final Grade) from the Assignment!
If you are a student from an English-speaking country, please feel free to contact us at [email protected] and we will provide you with an excellent writing service.
作为现存十年的代写服务机构，我们没有任何学术丑闻，我们保护顾客隐私、多元化辅导、写作、越来越多的小伙伴选择代写人为他们解决棘手的各类作业难题，保障GPA，为留学梦助力！ 我们的客服团队及写手老师总是能第一时间响应顾客的各类作业需求，有些人即使有重要的事甚至带伤上场协助考试。Final季，忙的时候一天十几场考试还在继续坚持着，我知道，他们明明可以不用这么辛苦的…但是他们为了坚守承诺，为了另一端屏幕外的那一份期望，他们没有选择退缩、时刻为同学们提供最好的！这么有温度的代写还不添加备用一下？WX/QQ： 5757940